Command-line tool#

clop#

Group command.

clop [OPTIONS] COMMAND [ARGS]...

Options

--version#

Show the version and exit.

eq1#

Model Kd dependence on pH.

clop eq1 [OPTIONS] KD1 PKA PH

Arguments

KD1#

Required argument

PKA#

Required argument

PH#

Required argument

fit-titration#

Fit Titration group command.

fit-titration [OPTIONS] COMMAND [ARGS]...

Options

--version#

Show the version and exit.

-v, --verbose#

Verbosity of messages.

-o, --out <out>#

Output folder.

--is-ph, --no-is-ph#

Concentrations are pH.

Default:

True

glob#

Update old glob fit of multiple datasets.

fit-titration glob [OPTIONS] FILE

Options

-b, --boot <boot>#

Number of booting iterations.

--weight, --no-weight#

Use residue weights.

Default:

True

Arguments

FILE#

Required argument

spec#

Update old svd or band fit of titration spectra.

fit-titration spec [OPTIONS] CSV_F NOTE_F

Options

-b, --band <band>#

Integration interval from <1> to <2>

Arguments

CSV_F#

Required argument

NOTE_F#

Required argument

ppr#

Parse Plate Reader ppr group command.

ppr [OPTIONS] COMMAND [ARGS]...

Options

--version#

Show the version and exit.

-v, --verbose#

Verbosity of messages.

-o, --out <out>#

Output folder.

enspire#

Save spectra as csv tables from EnSpire xls file.

ppr enspire [OPTIONS] CSV_F [NOTE_F]

Options

-b <bands>#

Label and band interval (format: LABEL LOWER UPPER)

Arguments

CSV_F#

Required argument

NOTE_F#

Optional argument

tecan#

Convert a list of Tecan-exported excel files into titrations.

LIST_FILE : List of Tecan files and concentration values.

Saves titrations as .dat files and fits all wells using 2 labels. The function produces:

  • K plot

  • csv tables for all labelblocks and global fittings.

Note: Buffer is always subtracted if scheme indicates buffer well positions.

ppr tecan [OPTIONS] LIST_FILE

Options

--cl <cl>#

Cl titration: [Cl] (mM) of added aliquots.

--bg#

Subtract buffer (from scheme.txt).

--bg-adj#

Adjust bg to avoid negative value.

--dil#

Apply dilution correction.

--nrm#

Normalize using metadata.

--bg-mth <bg_mth>#

Method for bg.

Default:

'mean'

--sch <sch>#

Plate scheme (buffers CTRs).

--add <add>#

Initial volume and additions.

--all#

Export (fit) all data combinations.

--lim <lim>#

Range MIN, MAX of plot_K.

-t, --title <title>#

Title for plots.

--fit, --no-fit#

Perform also fit.

Default:

True

--png, --no-png#

Export png files.

Default:

True

--mcmc, --no-mcmc#

Run MCMC sampling.

Default:

False

Arguments

LIST_FILE#

Required argument

note_to_csv#

Convert a tab-separated data file into a CSV file.

note_to_csv [OPTIONS] NOTE

Options

-o, --output <output>#

Output CSV file.

-l, --labels <labels>#

Labels to be appended.

-t, --temp <temp>#

Temperature to be appended.

Arguments

NOTE#

Required argument